Technology and Applications

ZellChip's technology relates to a biomicrofluidic device comprising fluid ports, fluid channels, and particle retention structures. Reagents and particles can be delivered to the microfluidic device through the fluid ports and the fluid channels. The technology also relates to methods of using the biomicrofluidic device to separate a single-cell from a group of cells, to culture a cell, to monitor, observe, measure, or record a biological parameter of a single-cell. Applications for Single Cell Biochip

      - Select single-cells such as prostate cancer cells or muscle cells for observation

      - Observe and image single-cells such as leukemia cells

      - Measure changes in single-cells using your own reagents (fluorescent drugs and chemical compounds

Applications for Single Cell Biochip Kit

      - Select and image single-cells such as leukemia cells

      - Image or measure reagent uptake in single-cells using a provided fluorescent probe

      - Image or measure uptake enhancement in single-cells using provided multidrug resistant (MDR) inhibitors or uptake enhancers

Applications for Single Cell Bioanalyzer:

      - Measure in real-time the uptake of reagents in single-cells

      - Measure the uptake enhancement in single-cells due to MDR inhibitors or uptake enhancers

      - Measure the MDR reversal of patient cells due to well-known P-gp inhibitors such as cyclosporine A

      - Perform real-time correction for background fluctuations due to reagent switching

      - Analyze uptake data and extract kinetic parameters for the uptake process

Possible Applications of Single Cell Bioanalysis

      - Analysis of blood cancer cells: leukemia cells, lymphoma cells

      - Analysis of solid cancer cells: melanoma cells, prostate cancer cells, ovarian cancer cells, breast cancer cells, lung cancer cells

      - chemotherapeutic drug testing: daunorubicin, doxorubicin, paclitaxel

      - enhancer testing: verapamil, cyclosporine A, MK571, fumitremorgin C

      - targeted agent testing: gefitinib

      - herbal compound testing: ginseng, reishi

      - tests for cardiovascular diseases drugs using heart cells

      - tests for osteoporosis drugs using bone cells

      - tests of drugs for mental illness, neurodegenerate diseases (drugs to cross the Blood Brain Barrier or BBB)

Literatures

1. Drug accumulation into single drug-sensitive and drug-resistant prostate cancer cells conducted on the single cell bioanalyzer presented at ASME Intl Mech. Engineering Congress & Exposition in Canada.

2. Drug resistance of a single mouse melanoma cell and its interaction with a mouse dendritic cell studied using the microfluidic single cell bioanalyzer presented at 6th International Symposium on Microchemistry and Microsystems in Singapore.

3. Drug Accumulation in Single Drug-Sensitive and Drug-Resistant Ovarian Cancer Cells and Acute Myeloid Leukemia Cells Conducted in a Single Cell Bioanalyzer presented at Lab-on-a-Chip European Congress in Germany.

4. Microfluidic Single Cell Bioanalysis to Measure Drug Uptake on Single Multidrug Resistant Cancer Cell as Enhanced by Ginsenoside Rg3(Hong Kong collaboration)

5. Same-single-cell analysis using the microfluidic biochip to reveal drug accumulation enhancement (Canadian collaboration)

6. Label-free isolation of a prostate cancer cell among blood cells and the single-cell measurement of drug accumulation using an integrated microfluidic chip(Australian collaboration)

7. Single-Cell Measurements for Multidrug Resistance in Heterogeneous Acute Myeloid Leukemia Patient Samples (BCCA and Australian collaborations)

8. Determination of Multidrug Resistance in Different Ovarian Cancer Cells using a Single-cell Bioanalyzer (US Collaboration)